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Regeneration of plantlets from mesophyll protoplasts ofBrassica juncea(L.) Czern

โœ Scribed by Gouri Chatterjee; Samir R. Sikdar; Srabani Das; S. K. Sen

Publisher
Springer
Year
1985
Tongue
English
Weight
803 KB
Volume
4
Category
Article
ISSN
0721-7714

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Protoplasts derived from hypocotyls of seedlings grown on half-strength MS medium containing 1% sucrose were cultured at a density of 5 x 104 ml ~ in Kao's medium supplemented with 1.0 mg 1 ~ 2,4-D, 0.1 mg 1l NAA and 0.5mgl ~ zeatin riboside. After three days of culture in darkness at 25 + IยฐC, cult