We compare the phosphorylation of 6-3H-TdR and 2-14C-TdR to TMP, TDP and TTP by tissue extracts and to T M P by purified kiizase pre~aratioiz~. The rate of'pho~phory~ation of'& 3H-TdR is only 0.6 to 0.8 that of 2-14C-TdR. The same isotopic discrimination exists between methyl-labelled thymidines. In
Radiolysis of 14C- and 3H-labelled thymidine
✍ Scribed by J. Kopoldová; V. Dedkǒvá
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- French
- Weight
- 519 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
10^−3^M oxygenated aqueous solutions of thymidine preparations, labelled with ^14^C and ^3^H in various positions were gammairradiated. Radiation produats formed were followed in dependenae of irradiation doses. Their yields were confronted with produats formed during the autoradiolysis of long time stored tritiated thymidine preparations, and results obtained Were disaussed.
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