The activity of L-glutamic acid decarboxylase (GAD) is commonly estimated by several radiometric methods, whereas a fluorimetric assay based on an enzymatic formation of NADPH as described by Y. Okada and C. Shimada [(1975) Brain Res. 98, 202-206] has been given little attention in biochemical and p
Radiochemical assay for glutaminase and glutamic acid decarboxylase
β Scribed by Alexander W. Wood; Mary E. McCrea; J.Edwin Seegmiller
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 378 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The action of glutamic-oxaloacetic transaminase on aspartate labeled with tritium in position 2 leads to the release of the label as tritiated water. The incubation mixture is applied to a small column of Dowex-l-OH. The labeled substrate is retained and the tritiated water is washed into a scintill
Malonyl-CoA decarboxylase is the main route for the disposal of malonyl-CoA, the key metabolite in the regulation of mitochondrial fatty acid oxidation. We have developed a simple and sensitive radiochemical assay to determine malonyl-CoA decarboxylase activity. The decarboxylation of [2-14C]malonyl
We report the presence of serum autoantibodies directed against glutamic acid decarboxylase in a patient with epilepsy and palatal myoclonus not associated with brain lesions. Glutamic acid decarboxylase antibody reactivity was dependent on the presence of carboxy-terminal amino acids, similar to th
## Abstract We tested whether transfer of the gene coding for glutamic acid decarboxylase to dorsal root ganglion using a herpes simplex virus vector to achieve release of GABA in dorsal horn would attenuate nociception in this condition. Subcutaneous inoculation of a replicationβdefective herpes s