A sensitive radiochemical assay for serum glutamic-oxaloacetic transaminase

✍ Scribed by L. Shuster; A. Bates; C.A. Hirsch

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 248 KB
• Volume: 86
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90791-1

✦ Synopsis

The action of glutamic-oxaloacetic transaminase on aspartate labeled with tritium in position 2 leads to the release of the label as tritiated water. The incubation mixture is applied to a small column of Dowex-l-OH. The labeled substrate is retained and the tritiated water is washed into a scintillation vial for counting. The radioactivity of the effluent is proportional to time of incubation and to enzyme concentration. Results obtained with human sera correlate very well with those obtained by a standard spectrophotometric assay. The radiochemical assay permits the use of serum samples of 10 ~1 or less. It has been used to study hepatotoxicity in mice.