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A radiochemical assay for N5-formyltetrahydrofolic acid (citrovorum factor) in serum and urine

✍ Scribed by Sheldon P. Rothenberg; Maria da Costa; Zoltan Rosenberg

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
384 KB
Volume
94
Category
Article
ISSN
0003-2697

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✦ Synopsis

N5-Methyltetrahydrofolate,

but not N5-formyltetrahydrofolate, can be measured in biological fluids by ligand-binding radioassay. Therefore, in order to measure N5-formyltetrahydrofolate by radioassay, it is chemically converted to N5-methyltetrahydrofolate by acidification followed by reduction with borohydride. By this method, 70-113% ofN5-formyltettahydrofolate added to serum and urine was recovered. The plasma clearance of the mixture of diastereoisomer of N 5-formyltetrahydrofolate (Leucovorin) following intravenous administration to two normal subjects was rapid for the first 30 min, but then plateaued and cleared very slowly over the next 90 min, most probably because of the accumulation of the inactive isomer which was slowly excreted in the urine during this time period.

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