𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Quantitative determination of acyl chain composition of subnanomole amounts of cellular long-chain acyl-coenzyme A esters

✍ Scribed by M.Renuka Prasad; John Sauter; William E.M. Lands

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
952 KB
Volume
162
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A procedure for the quantitative determination of the acyl chain composition of cellular long-chain acyl-CoA esters in subnanomole amounts is described. The abundant cellular lipids of samples are removed by extraction with organic solvents, and the proteins are precipitated from the aqueous phase by the addition of acetonitrile. The CoA thiolesters are adsorbed on neutral aluminum oxide and reduced with sodium borohydride to the corresponding alcohols that are then converted to t-butyldimethylsilyl ethers and analyzed quantitatively by gas chromatography. Saturated and unsaturated acyl chains behaved similarly throughout the procedure, and the common lipid esters do not interfere with the analysis of the CoA esters in the final assay procedure described. This simple and relatively rapid method is suitable for analyzing a large number of samples at a time.

πŸ“œ SIMILAR VOLUMES

Isolation and Quantitation of Long-Chain
Isolation and Quantitation of Long-Chain Acyl-Coenzyme A Esters in Brain Tissue by Solid-Phase Extraction
✍ J. Deutsch; E. Grange; S.I. Rapoport; A.D. Purdon πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 259 KB

Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and deter

A fast and versatile method for extracti
A fast and versatile method for extraction and quantitation of long-chain acyl-CoA esters from tissue: Content of individual long-chain acyl-CoA esters in various tissues from fed rat
✍ Jesper Rosendal; Jens Knudsen πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 513 KB

A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon

A specific and inexpensive assay of radi
A specific and inexpensive assay of radiolabeled long-chain acyl-coenzyme a in isolated hepatocytes
✍ Hedwig K. Stals; Peter E. Declercq πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 298 KB

A simple procedure for determining radiolabeled long-chain acyl-CoA levels in isolated rat hepatocytes has been developed. It consists of a classical extraction of long-chain acyl-CoAs after preliminary removal of the excess labeled free fatty acid. A two-step TLC purification ensures elimination of

Continuous Recording of Long-Chain Acyl-
Continuous Recording of Long-Chain Acyl-Coenzyme A Synthetase Activity Using Fluorescently Labeled Bovine Serum Albumin
✍ Erland J.F. Demant; Birthe T. NystrΓΈm πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 100 KB

The fluorescence-based long-chain fatty acid probe BSA-HCA (bovine serum albumin labeled with 7-hydroxycoumarin-4-acetic acid) is shown to respond to binding of long-chain acyl-CoA thioesters by quenching of the 450 nm fluorescence emission. As determined by spectrofluorometric titration, binding af