A fast and versatile method for extraction and quantitation of long-chain acyl-CoA esters from tissue: Content of individual long-chain acyl-CoA esters in various tissues from fed rat

✍ Scribed by Jesper Rosendal; Jens Knudsen

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 513 KB
• Volume: 207
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(92)90500-7

✦ Synopsis

A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammonium acetate). Reextraction of the dry residue after evaporation of extraction solvent results in low overall recoveries (20%). By adding 1 mg/ml acyl-CoA-binding protein to the extraction solvent the overall recovery was increased to 55%. The method is easy and fast to perform and is thereby suitable for analysis of a large number of samples. The advantages of the method over previously published methods are discussed.