Isolation and Quantitation of Long-Chain Acyl-Coenzyme A Esters in Brain Tissue by Solid-Phase Extraction

✍ Scribed by J. Deutsch; E. Grange; S.I. Rapoport; A.D. Purdon

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 259 KB
• Volume: 220
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1344

✦ Synopsis

Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acylCoA's. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 (\mathrm{nmol} / \mathrm{g})), palmitoyl-CoA ((6.0 \mathrm{nmol} / \mathrm{g})), stearoylCoA ((4.0 \mathrm{nmol} / \mathrm{g})), and linoleoyl- and arachidonoyl-CoA ((2.0 \mathrm{nmol} / \mathrm{g})) for a total concentration of (23 \mathrm{nmol} / \mathrm{g}) brain. 1994 Academic Press, Inc.