A protocol for the identification and estimation of short-chain esters of carnitine is described; it is useful for the diagnosis of acidemias. By this method, camitine esters in urine are converted to coenzyme A esters enzymatically with camitine acetyltransferase (CAT): short-chain acylcamitine + C
Determination of short-chain acyl-coenzyme A esters by high-performance liquid chromatography
β Scribed by Yoshitaka Hosokawa; Yoshiharu Shimomura; Robert A. Harris; Takayuki Ozawa
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 425 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method for determining tissue levels of Coenzyme A and various short-chain-length acyl-CoA derivatives using high-performance liquid chromatography is presented. Separation of the various compounds was accomplished using a reverse-phase Spherisorb ODS II, 5-pm Cl8 column. Mobile-phase solvents wer
An isocratic HPLC method was developed for the determination of coenzyme Q(10) (CoQ(10)) in human breast milk. After a single-step liquid-liquid extraction, the milk extract was injected directly into the HPLC system. The analytical method is based on pre-column inline treatment of CoQ(10). Chromato
Long-chain acyl-CoA esters were extracted from freeze-clamped livers of fed and fasted rats according to the method of Mancha et al. [M. Mancha, G. B. Stokes, and P. K. Stumpf (1975) Anal. Biochem. 68, 600-6081 and analyzed on a radially compressed Cis, 5 pm, reverse-phase column using a gradient sy
A simple, one-step method has been developed for the analysis of the components of egg phosphatidylcholine. The method allows, in one run, (a) determination of the total number of carbon atoms and double bonds in the acyl chains of individual components in the mixture and (b) unequivocal assignment
## Abstract This work suggests an HPLC method for qualitative and quantitative determination of __N__^Ξ΅^(2βaminoβ2βcarboxyethyl)βLβlysine (LAL). LAL was released from total hydrolysates of various proteins of animal origin and derivatized with dansyl chloride. The performance of two different colum