✦ LIBER ✦

A specific and inexpensive assay of radiolabeled long-chain acyl-coenzyme a in isolated hepatocytes

✍ Scribed by Hedwig K. Stals; Peter E. Declercq

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 298 KB
Volume: 202
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(92)90216-t

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✦ Synopsis

A simple procedure for determining radiolabeled long-chain acyl-CoA levels in isolated rat hepatocytes has been developed. It consists of a classical extraction of long-chain acyl-CoAs after preliminary removal of the excess labeled free fatty acid. A two-step TLC purification ensures elimination of long-chain acylcarnitine, the main interferent in most methods, and other common lipids. The purity of the acyl-CoA was confirmed by a second TLC system and by spectral analysis. Overall recovery was approximately 70%.

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