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Purification and Characterization of DNA-Dependent RNA Polymerase from Staphylococcus aureus

✍ Scribed by R. Deora; T.K. Misra


Book ID
115576315
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
381 KB
Volume
208
Category
Article
ISSN
0006-291X

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Purification of DNA-dependent RNA polyme
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A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a