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Purification of DNA-dependent RNA polymerase fromStreptomyces granaticolor

✍ Scribed by J. Spížek; P. Ryšavý; M. Klégr; J. Náprstek; J. Janeček

Book ID
112924803
Publisher
Springer Netherlands
Year
1983
Tongue
English
Weight
358 KB
Volume
28
Category
Article
ISSN
0015-5632

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✍ Grant A. Bitter 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 807 KB

A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a