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Purification and Properties of DNA-Dependent RNA Polymerases from Yeast

✍ Scribed by Helmut Ponta; Ulrike Ponta; Erhard Wintersberger


Book ID
115113246
Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
967 KB
Volume
29
Category
Article
ISSN
1432-1327

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Purification of DNA-dependent RNA polyme
✍ Grant A. Bitter πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 807 KB

A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a