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Purification and Properties of DNA-Dependent RNA-Polymerase from Bacillus stearothermophilus

✍ Scribed by E. Remold-O'Donnell; W. Zillig


Book ID
115112122
Publisher
John Wiley and Sons
Year
1969
Tongue
English
Weight
451 KB
Volume
7
Category
Article
ISSN
1432-1327

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Purification of DNA-dependent RNA polyme
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A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a