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Purification and characterization of a soluble DNA-dependent chloroplast RNA polymerase from Pisum sativum

โœ Scribed by Angelique S Boyer; Richard B Hallick

Book ID
117383815
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
348 KB
Volume
137
Category
Article
ISSN
0168-9452

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โœ Grant A. Bitter ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 807 KB

A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a