Pseudo-allosteric behavior of firefly luciferase
โ Scribed by Jeffrey Denburg; Marlene DeLuca
- Book ID
- 118850725
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 270 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0006-291X
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A DNA segment carrying the full-length, intronless firefly luciferase gene was inserted into the high expression secretion vector, pIN-Wr -ompA. Upon induction of gene expression, luciferase activity was detected in extracts prepared from periplasmic fractions. The results indicated that the OmpA s
Twelve naturally occurring nucleoside triphosphates have been examined as substrates and inhibitors of the light-producing reaction of firefly luciferase. Deoxyadenosine 5'-triphosphate was 1.7% as effective relative to ATP as a substrate, whereas all others tested were less than 0.1% as effective a