✦ LIBER ✦

Analytical applications of firefly luciferase

✍ Scribed by Jon C. Wannlund

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 407 KB
Volume: 2
Category: Article
ISSN: 0165-9936
DOI: 10.1016/0165-9936(83)87066-6

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Secretion of firefly luciferase inE.coli

✍ Jian-Rong Lu; Jian Yang; Zhen-Hua Jin 📂 Article 📅 1993 🏛 Springer Netherlands 🌐 English ⚖ 346 KB

A DNA segment carrying the full-length, intronless firefly luciferase gene was inserted into the high expression secretion vector, pIN-Wr -ompA. Upon induction of gene expression, luciferase activity was detected in extracts prepared from periplasmic fractions. The results indicated that the OmpA s

New approaches to the preparation and ap

New approaches to the preparation and application of firefly luciferase

✍ Filippova, N. Yu. ;Dukhovich, A. F. ;Ugarova, N. N. 📂 Article 📅 1989 🏛 John Wiley and Sons ⚖ 279 KB 👁 1 views

Atypical kinetics of immobilized firefly

Atypical kinetics of immobilized firefly luciferase

✍ Loïc J. Blum; Pierre R. Coulet 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 488 KB

Nucleoside triphosphate specificity of f

Nucleoside triphosphate specificity of firefly luciferase

✍ James D. Moyer; J.Frank Henderson 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 225 KB

Twelve naturally occurring nucleoside triphosphates have been examined as substrates and inhibitors of the light-producing reaction of firefly luciferase. Deoxyadenosine 5'-triphosphate was 1.7% as effective relative to ATP as a substrate, whereas all others tested were less than 0.1% as effective a

The effect of detergents on firefly luci

The effect of detergents on firefly luciferase reactions

✍ Simpson, W. J. ;Hammond, J. R. M. 📂 Article 📅 1991 🏛 John Wiley and Sons ⚖ 811 KB

The reaction rate of ATP-limited firefly luciferase-catalysed reactions, is affected by the presence o f detergents. Anionic detergents inhibit luciferase activity without causing significant enzyme inactivation during the reaction. Cationic detergents in- crease reaction rate several-fold w i t h a

Enhancement of firefly luciferase activi

Enhancement of firefly luciferase activity by cytidine nucleotides

✍ Sharon R. Ford; Marliese S. Hall; Franklin R. Leach 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 931 KB

The temporal pattern of light production by firefly luciferase depends on the ATP concentration. With low concentrations of ATP a constant production of light occurred while at high concentrations of ATP (greater than 10 microM) there was a flash of light followed by a decline in light production. T