Atypical kinetics of immobilized firefly luciferase
✍ Scribed by Loïc J. Blum; Pierre R. Coulet
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 488 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0006-3592
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Biochemical properties, spectral parameters of bioluminescence and reaction kinetics for Luciola mingrelica firefly luciferase are described and analysed. The kinetic scheme of the enzymatic process is proposed and discussed. Allosteric regulation of luciferase activity by ATP and its analogues is c
A DNA segment carrying the full-length, intronless firefly luciferase gene was inserted into the high expression secretion vector, pIN-Wr -ompA. Upon induction of gene expression, luciferase activity was detected in extracts prepared from periplasmic fractions. The results indicated that the OmpA s