The bioluminescent reaction catalysed by firefly luciferase has become widely established as an outstanding analytical system for assay of ATP. When used in solution, luciferase is unstable and cannot be re-used, a problem that can be partially circumvented by immobilizing the enzyme on solid substr
β¦ LIBER β¦
Immobilization of firefly luciferase on glass rods: Properties of the immobilized enzyme
β Scribed by Y. Lee; I. Jablonski; M. DeLuca
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 296 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0003-2697
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Whole cell immobilization of Lactobacillus sp. cells for D-glucose isomerase activity was done by taking well washed cell slurries of bacteria, coating them on rough surfaces of glass rods or glass slides, drying them at 50 "C to 55 "C under infra red lamp and finally treating with polyvinyl alcohol