✦ LIBER ✦

Whole cell immobilization of D-glucose isomerase enzyme on glass support

✍ Scribed by Dr. G. L. Shukla; K. A. Prabhu

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 252 KB
Volume: 28
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620280708

No coin nor oath required. For personal study only.

✦ Synopsis

Whole cell immobilization of Lactobacillus sp. cells for D-glucose isomerase activity was done by taking well washed cell slurries of bacteria, coating them on rough surfaces of glass rods or glass slides, drying them at 50 "C to 55 "C under infra red lamp and finally treating with polyvinyl alcohol. The immobilized glucose isomerase activity of cells showed remarkable change in pH optimum, thermal stability, K,,, and V,,, values as compared to free enzyme of cells. The immobilized enzyme system was employed in the conversion of glucose to fructose using concentrates of amylase-treated corn starch and cellulase-treated bagasse cellulose.

📜 SIMILAR VOLUMES

Mass-transfer effects on the rate of iso

Mass-transfer effects on the rate of isomerization of D-glucose into D-fructose, catalyzed by whole-cell immobilized glucose isomerase

✍ J. G. Boersma; K. Vellenga; H. G. J. de Wilt; G. E. H. Joosten 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 582 KB

The isomerization of D-glucose into D-fr

The isomerization of D-glucose into D-fructose catalyzed by whole-cell immobilized glucose isomerase. The dependence of the intrinsic rate of reaction on substrate concentration, pH, and temperature

✍ A. Kikkert; K. Vellenga; H. G. J. de Wilt; G. E. H. Joosten 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 538 KB

Fundamental studies of glucose oxidase i

Fundamental studies of glucose oxidase immobilization on controlled pore glass

✍ Md. M. Hossain; D. D. Do 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 774 KB

Kinetics of the isomerization of D-gluco

Kinetics of the isomerization of D-glucose into D-fructose catalyzed by glucose isomerase containing Arthrobacter cells in immobilized and nonimmobilized form

✍ M. A. Van Keulen; K. Vellenga; G. E. H. Joosten 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 565 KB

Effects of immobilization on the kinetic

Effects of immobilization on the kinetics of enzyme-catalyzed reactions. I. Glucose oxidase in a recirculation reactor system

✍ K. B. Ramachandran; D. D. Perlmutter 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 652 KB

Immobilization of oxydoreductases on ino

Immobilization of oxydoreductases on inorganic supports based on alumina: The role of mutual correspondence of enzyme-support hydrophobic–hydrophilic characters

✍ V. D. Sokolovskii; G. A. Kovalenko 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 332 KB 👁 2 views