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Secretion of firefly luciferase inE.coli

โœ Scribed by Jian-Rong Lu; Jian Yang; Zhen-Hua Jin

Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
346 KB
Volume
15
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

A DNA segment carrying the full-length, intronless firefly luciferase gene was inserted into the high expression secretion vector, pIN-Wr -ompA.

Upon induction of gene expression, luciferase activity was detected in extracts prepared from periplasmic fractions. The results indicated that the OmpA signal peptide was able to direct secretion of firefly luciferase across the cytoplasmic membrane. This has important implications for using this luciferase as a reporter in studying protein export and targeting.

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