๐”– Bobbio Scriptorium
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Nucleoside triphosphate specificity of firefly luciferase

โœ Scribed by James D. Moyer; J.Frank Henderson

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
225 KB
Volume
131
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Twelve naturally occurring nucleoside triphosphates have been examined as substrates and inhibitors of the light-producing reaction of firefly luciferase. Deoxyadenosine 5'-triphosphate was 1.7% as effective relative to ATP as a substrate, whereas all others tested were less than 0.1% as effective as ATP. At concentrations normally present in mammalian cell extracts no interference with ATP measurements results from these nucleotides.

๐Ÿ“œ SIMILAR VOLUMES

Secretion of firefly luciferase inE.coli
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โœ Jian-Rong Lu; Jian Yang; Zhen-Hua Jin ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 346 KB

A DNA segment carrying the full-length, intronless firefly luciferase gene was inserted into the high expression secretion vector, pIN-Wr -ompA. Upon induction of gene expression, luciferase activity was detected in extracts prepared from periplasmic fractions. The results indicated that the OmpA s