A unique rapid method for assaying specific binding reactions with ligandcofactor conjugates and a bioluminescent reaction is described. Biotin and 2,4dinitrofluorobenzene were coupled covalently to the free amino residue of nicotinamide 6-(2-aminoethylamino) purine dinucleotide (AENAD) to produce t
โฆ LIBER โฆ
Specific protein-binding reactions monitored with ligand-ATP conjugates and firefly luciferase
โ Scribed by Robert J. Carrico; Kwok-Kam Yeung; Hartmut R. Schroeder; Robert C. Boguslaski; Robert T. Buckler; James E. Christner
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 951 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Specific binding reactions monitored wit
โ
Hartmut R. Schroeder; Robert J. Carrico; Robert C. Boguslaski; James E. Christne
๐
Article
๐
1976
๐
Elsevier Science
๐
English
โ 572 KB
Specific protein-binding reactions monit
โ
J.F. Burd; R.J. Carrico; M.C. Fetter; R.T. Buckler; R.D. Johnson; R.C. Boguslask
๐
Article
๐
1977
๐
Elsevier Science
๐
English
โ 678 KB
Specific protein-binding reactions were monitored utilizing ligand-fluorescent dye conjugates which were substrates for porcine esterase. Biotin was coupled directly to umbelliferone through an ester bond, while 2,4-dinitrophenyl derivatives were conjugated to fluorescein via an ester linkage. Hydro