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Specific protein-binding reactions monitored with ligand-ATP conjugates and firefly luciferase

โœ Scribed by Robert J. Carrico; Kwok-Kam Yeung; Hartmut R. Schroeder; Robert C. Boguslaski; Robert T. Buckler; James E. Christner


Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
951 KB
Volume
76
Category
Article
ISSN
0003-2697

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Specific binding reactions monitored wit
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A unique rapid method for assaying specific binding reactions with ligandcofactor conjugates and a bioluminescent reaction is described. Biotin and 2,4dinitrofluorobenzene were coupled covalently to the free amino residue of nicotinamide 6-(2-aminoethylamino) purine dinucleotide (AENAD) to produce t

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Specific protein-binding reactions were monitored utilizing ligand-fluorescent dye conjugates which were substrates for porcine esterase. Biotin was coupled directly to umbelliferone through an ester bond, while 2,4-dinitrophenyl derivatives were conjugated to fluorescein via an ester linkage. Hydro