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Properties of a sarcoma-growth-factor-like peptide from cells transformed by a temperature-sensitive sarcoma virus

✍ Scribed by Joseph E. De Larco; Yvette A. Preston; George J. Todaro

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
869 KB
Volume
109
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Serum‐free conditioned media was collected from three sarcoma virus‐transformed cell lines and an untransformed cell line. All three virally transformed lines produced and released growth factors into their serum‐free media. The major activity in all cases, whether the cells were transformed by Moloney sarcoma virus (MSV) or Kirsten sarcoma virus (KiSV), or whether they were mouse or rat, was a sarcoma‐growth‐factor (SGF)‐like activity with an apparent molecular weight of 10,000. The SGF‐like pools from a Moloney sarcoma virus‐transformed mouse 3T3 cell and a Kirsten sarcoma virus‐transformed NRK cell were further purified by carboxymethyl cellulose chromatography. The elution profiles of these peptides were very similar. The serum‐free conditioned media from the untransformed cells showed no detectable growth stimulating activity. The temperature sensitivity of an SGF‐like growth factor from the supernate of a NRK cell transformed by a wild‐type Kirsten sarcoma virus (KiSV) was compared with that of the SGF‐like activity from the supernates of a NRK cell transformed by a ts‐mutant of KiSV that is temperature sensitive with respect to transformation (ts‐371 Cl 5). Neither the cells transformed by the wild‐type sarcoma virus nor those transformed by the temperature sensitive virus released a SGF‐like activity that was temperature sensitive under the conditions of the assays.

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Abbreviations: ECM, extracellular matrix; SV40, simian virus 40; a-SMA, a-smooth cells were isolated from a single animal to develop homogeneous muscle actin; TGF-b1, transforming growth factor b1; PCR, polymerase chain reaction.