Temperature-dependent alterations in 2-deoxyglucose uptake in rat cells transformed by a cold-sensitive murine sarcoma virus mutant

✍ Scribed by J. T. May; K. D. Somers; Saul Kit

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: French
• Weight: 500 KB
• Volume: 11
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910110215

✦ Synopsis

Abstract

Cells transformed by Moloney sarcoma virus (MSV) take up 2‐deoxyglucose at a faster rate at 39° C than uninfected or Moloney leukemia virus (MoLV)—infected normal rat kidney (NRK) cells. In a sarcoma‐virus‐transformed cell line, NRK (MSV‐lb), whose transformed phenotype is expressed at 39° C (permissive) but not at 33° C (non‐permissive), the stimulation of 2‐deoxyglucose uptake at 39° C is temperature dependent; the increase is observed when cells are grown at 39° C but not at 33 C. When these cells were shifted from the non‐permissive to the permissive temperature, the uptake increased from a rate near that of uninfected cells to a rate half that of NRK cells infected with Moloney sarcoma‐leukemia complex (MSV‐MoLV). The reverse change occurred when the cells were shifted from the permissive to the non‐permissive temperature. Thus in a sarcoma virus‐infected rat cell line where the maintenance of the transformed state is dependent on a cold‐sensitive viral function, the uptake of 2‐deoxyglucose is reduced in the cold‐sensitive cells and correlated with the expression of the transformed phenotype.