The levels of simian virus 40 (SV40) large T antigen in a tsA-transformed mouse macrophage line at the permissive (33 degrees C) and the nonpermissive (39 degrees C) temperature were examined by immunofluorescence, sodium dodecylsulfate-polyacrylamide gel electrophoresis, complement fixation, and en
Cell growth and differentiation of a novel mouse Ito (fat-storing) cell line transformed by a temperature-sensitive mutant of simian virus 40
✍ Scribed by Y Kitamura; T Tanigawa; T Katsumoto; K Tomita; H Wang; K Hirai; K Ichihara; T Terada
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 990 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
Abbreviations: ECM, extracellular matrix; SV40, simian virus 40; a-SMA, a-smooth cells were isolated from a single animal to develop homogeneous muscle actin; TGF-b1, transforming growth factor b1; PCR, polymerase chain reaction.
📜 SIMILAR VOLUMES
It was shown previously that mouse bone marrow cells transformed by simian virus 40 (SV40) show a reversible cell density-dependent phenotypic transition between the nonmacrophage (rapidly growing) and the macrophage (stationary) states; cells in low-density cultures are in the growing phase, expres
Cultures of mouse macrophage cell lines transformed by wild-type or the tsA640 mutant of simian virus 40 (SV40) show a reversible phenotypic transition between the nonmacrophage (proliferating phase) and the macrophage (stationary phase) states (Takayama, 1980; Tanigawa et al., 1983). Distribution o
## Abstract Hamster cells (BHK21/13) transformed at the permissive temperature by a temperature‐sensitive mutant (FU 19) of the Schmidt‐Ruppin strain of Rous sarcoma virus exhibited a temperature‐dependent expression of virus‐induced surface antigen (VISA) as assayed by mixed haemadsorption test. T
Four temperature-sensitive (ts) mutants of rat 3Y1 fibroblasts, representing independent complementation groups, cease to proliferate predominantly with a 2n DNA content, at the restrictive temperature (39.8OC)(temperature arrest) or at the permissive temperature (33.8OC) at a confluent cell density