Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then
Production of germ line chimera by transfer of primordial germ cells in the domestic chicken (Gallusdomesticus)
โ Scribed by A. Tajima; M. Naito; Y. Yasuda; T. Kuwana
- Book ID
- 118379869
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 667 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0093-691X
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## Abstract Intrinsic primordial germ cells (PGCs) from stage 27 (5โdayโold) chick embryonic germinal ridges were cultured in vitro for a further 5 days, and shown to proliferate on stroma cells derived from the germinal ridge. To determine whether these cultured PGCs could colonize and contribute
Primordial germ cells (PGCs) from stage 27 (5.5-day-old) Korean native ogol chicken embryonic germinal ridges were cultured in vitro for 5 days. As in in vivo culture, these cultured PGCs were expected to have already passed beyond the migration stage. Approximately 200 of these PGCs were transferre