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Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos

โœ Scribed by Mi A. Kim; Tae Sub Park; Jin Nam Kim; Hyun Jeong Park; Young Mok Lee; Tamao Ono; Jeong Mook Lim; Jae Yong Han


Book ID
116910264
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
211 KB
Volume
63
Category
Article
ISSN
0093-691X

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Production of germ-line chimeras by tran
โœ Tajima, Atsushi; Naito, Mitsuru; Yasuda, Yoshiaki; Kuwana, Takashi ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 69 KB ๐Ÿ‘ 2 views

Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then