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Production of germline chimeras by transfer of chicken gonadal primordial germ cells maintained in vitro for an extended period

โœ Scribed by Jae Yong Han; Tae Sub Park; Yeong Ho Hong; Dong Kee Jeong; Jin Nam Kim; Ki Dong Kim; Jeong Mook Lim


Book ID
117270694
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
229 KB
Volume
58
Category
Article
ISSN
0093-691X

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Production of germ-line chimeras by tran
โœ Tajima, Atsushi; Naito, Mitsuru; Yasuda, Yoshiaki; Kuwana, Takashi ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 69 KB ๐Ÿ‘ 2 views

Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then