Production of germ-line chimeras by tran
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Tajima, Atsushi; Naito, Mitsuru; Yasuda, Yoshiaki; Kuwana, Takashi
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Article
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1998
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John Wiley and Sons
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English
โ 69 KB
๐ 2 views
Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then