Production of germ-line chimeras by the transfer of cryopreserved gonadal germ cells collected from 7- and 9-day-old chick embryos

Production of germ-line chimeras by tran

Production of germ-line chimeras by transfer of cryopreserved gonadal primordial germ cells (gPGCs) in chicken

✍ Tajima, Atsushi; Naito, Mitsuru; Yasuda, Yoshiaki; Kuwana, Takashi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 69 KB 👁 2 views

Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1°C/min until the temperature reached -80°C; cells were then