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Germ-line chimeras can be produced by embryonic stem cell transfer in the chicken

โœ Scribed by J.N. Petitte; Liu Guodong; A.M.Verrinder Gibbins; R.J. Etches


Book ID
113395495
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
101 KB
Volume
27
Category
Article
ISSN
0922-3371

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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then