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Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane

✍ Scribed by Youji Shimazaki; Takahiro Kuroda

Book ID
106364455
Publisher
Springer Netherlands
Year
2009
Tongue
English
Weight
184 KB
Volume
31
Category
Article
ISSN
0141-5492

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## Abstract After separation by microscale non‐denaturing two‐dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t