Analysis of trypsin inhibition activity in human plasma proteins after separation by non-denaturing two-dimensional electrophoresis
β Scribed by Shimazaki, Youji; Michhiro, Madoka
- Book ID
- 122667911
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 701 KB
- Volume
- 425
- Category
- Article
- ISSN
- 0009-8981
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## Abstract After separation by microscale nonβdenaturing twoβdimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t
To examine the activities and identity of enzymes associated with organelles such as microsomes and mitochondria, proteins from mouse liver were extracted using the non-ionic detergents Nonidet P-40 (NP-40), polyoxyethylene sorbitan monooleate (Tween 80), polyoxyethylene isooctylphenyl ester (Triton