## Abstract After separation by microscale non‐denaturing two‐dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t
✦ LIBER ✦
Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis
✍ Scribed by Youji Shimazaki; Sono Watanabe
- Book ID
- 116347838
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 324 KB
- Volume
- 390
- Category
- Article
- ISSN
- 0009-8981
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To examine the activities and identity of enzymes associated with organelles such as microsomes and mitochondria, proteins from mouse liver were extracted using the non-ionic detergents Nonidet P-40 (NP-40), polyoxyethylene sorbitan monooleate (Tween 80), polyoxyethylene isooctylphenyl ester (Triton