✦ LIBER ✦

Direct analysis of retinal dehydrogenase activity on an electroblotting membrane following separation by non-denaturing two-dimensional electrophoresis

✍ Scribed by Youji Shimazaki; Takahiro Kuroda

Book ID: 108159793
Publisher: Elsevier Science
Year: 2007
Tongue: English
Weight: 465 KB
Volume: 860
Category: Article
ISSN: 1873-376X
DOI: 10.1016/j.jchromb.2007.10.028

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Analysis of lipid hydrolytic activity by

Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis

✍ Youji Shimazaki; Masayuki Miyamoto 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 190 KB

## Abstract After separation by microscale non‐denaturing two‐dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t

Simultaneous immobilization of dehydroge

Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis

✍ Youji Shimazaki; Mariko Kadota 📂 Article 📅 2008 🏛 Elsevier Science 🌐 English ⚖ 865 KB

Production of enzyme reactors after sepa

Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane

✍ Youji Shimazaki; Takahiro Kuroda 📂 Article 📅 2009 🏛 Springer Netherlands 🌐 English ⚖ 184 KB

Analysis of trypsin inhibition activity

Analysis of trypsin inhibition activity in human plasma proteins after separation by non-denaturing two-dimensional electrophoresis

✍ Shimazaki, Youji; Michhiro, Madoka 📂 Article 📅 2013 🏛 Elsevier Science 🌐 English ⚖ 701 KB

Silver staining of an esterase compatibl

Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis

✍ Youji Shimazaki; Sono Watanabe 📂 Article 📅 2008 🏛 Elsevier Science 🌐 English ⚖ 324 KB

Detection of activity and mass spectrome

Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents

✍ Youji Shimazaki; Takashi Manabe 📂 Article 📅 2005 🏛 Elsevier Science 🌐 English ⚖ 808 KB

To examine the activities and identity of enzymes associated with organelles such as microsomes and mitochondria, proteins from mouse liver were extracted using the non-ionic detergents Nonidet P-40 (NP-40), polyoxyethylene sorbitan monooleate (Tween 80), polyoxyethylene isooctylphenyl ester (Triton