Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis
β Scribed by Youji Shimazaki; Mariko Kadota
- Book ID
- 108047964
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 865 KB
- Volume
- 618
- Category
- Article
- ISSN
- 0003-2670
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To examine the activities and identity of enzymes associated with organelles such as microsomes and mitochondria, proteins from mouse liver were extracted using the non-ionic detergents Nonidet P-40 (NP-40), polyoxyethylene sorbitan monooleate (Tween 80), polyoxyethylene isooctylphenyl ester (Triton
## Abstract After separation by microscale nonβdenaturing twoβdimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t