Purification and properties of a-amylase from Micrococcus variuns ADEYEMI I . ADELEYE i Rcc?ird I 0 .4u</i~s/ 19cYYiArccy,tetl 6 FdJruciry I9901 An extraccllular cx-amylase from Micrococcus ivxinns was partially purified (63 fold) by ammonium sulphate precipitation followed by dialysis and separate
Production and control of extracellular α-amylase in Micrococcus varians
✍ Scribed by Dr. Adeyemi I. Adeleye
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 313 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
An extracellular α‐amylase was induced in cultures of Micrococcus varians during growth in a liquid medium containing starch as sole carbon source. Synthesis of this enzyme was repressed by the addition of glucose or fructose to starch metabolizing cells and was induced in a glucose or fructose metabolizing culture by the addition of starch.
Glycine was found to enhance growth and production of α‐amylase while the least growth and enzyme activity was recorded in medium containing ammonium sulphate as nitrogen source.
📜 SIMILAR VOLUMES
Studies on the a-amylase synthesis were carried out with two strains of Bacillus lichenijormis: a mutant strain 44MB82 and its selected variant 44MB82-G. The cells were cultivated in a nutrient medium containing glucose or citrate as a carbon source. The results obtained indicated that a-amylase was
The kinetics of growth, cxtraccllular a-amylase formation and pool sizes of gunnosine polyphosphates (p)ppGpp and adenosine phosphates (ATP and AMP) were determined during discontinuous cultivation of Bacillus subtilis 44. The results indicate a positive involvemcnt of (p)ppGpp in the regulation of
Fed-batch culture with controlled L-amino acid composition was performed to improve production of a recombinant gene product in Bacillus brevis. The maximum recombinant protein (a-amylase) level and specific activity increased from 5.14 kU/mL and 0.77 kU/mg dry cell in conventional fed-batch culture