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Effect of glucose and citrate on α-amylase production in Bacillus licheniformis

✍ Scribed by Dr. Alexandra Tonkova

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
338 KB
Volume
31
Category
Article
ISSN
0233-111X

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✦ Synopsis

Studies on the a-amylase synthesis were carried out with two strains of Bacillus lichenijormis: a mutant strain 44MB82 and its selected variant 44MB82-G. The cells were cultivated in a nutrient medium containing glucose or citrate as a carbon source. The results obtained indicated that a-amylase was produced in both strains without inducers in the medium. The variant strain 44MB82-G, which synthesized a-amylase in the presence of 6% glucose, showed 58-fold increased a-amylase activity compared to strain 44MB82 grown in the presence of 2% glucose. When cells of both strains were cultivated in a medium with 1% citrate, a-amylase activity of the variant strain was 5 times higher than that of strain 44MB82. The effects of starch and ATP on a-amylase synthesis are also discussed.

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