Preparation of very high specific activity [3H]-methyl acetimidate for protein labeling
β Scribed by T.Y. Toribara
- Publisher
- Elsevier Science
- Year
- 1978
- Weight
- 207 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0020-708X
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π SIMILAR VOLUMES
High-specific-activity D-[3-3H]pantothenic acid (5 Ci/mmol) was prepared from commercially available beta-[3-3H]alanine employing Escherichia coli strain DV1 (panD2 pan F1). This strain is defective in beta-alanine synthesis and pantothenate uptake, and under appropriate growth conditions converted
Isotopic exchange of hydrogen in a system of s~lvent-water-~H was used to prepare 5-Juoroorotic a ~i d -~H ( G ) which was decarboxylated and the hydrogen-3H removed from labile bonds to yield 5'-Juorouracil-6-3H. It was found in tracer experiments that a suitable solvent for the isotopic exchange o
The preparation of the 61-specific ligand 7-benzylidenenaltrexone (BNTX), labeled with tritium at high specific activity (14.4 Ciimmol) was prepared in 33% yield and >98% purity by the aldol condensation of high specific activity [15,1 6-3H]naltrexone with benzaldehyde at high dilution.
## Abstract Tritiated hydrocarbons may be prepared by Kolbe electrolysis of carboxylic acids which have been labelled by catalytic exchange in tritiated water.