✦ LIBER ✦

Preparation of polyacrylamide gel-filled capillaries by photopolymerization for capillary electrophoresis

✍ Scribed by Gerard J. M. Bruin; Tianlin Wang; Xiaoma Xu; Johan C. Kraak; Hans Poppe

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 623 KB
Volume: 4
Category: Article
ISSN: 1040-7685
DOI: 10.1002/mcs.1220040512

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A new preparation method for polyacrylamide gels in fused silica capillaries is described. In this method use is made of riboflavin as a light sensitive initiator for the polymerization reaction. Also, results from measurements on the diffusion coefficients of four oligonucleotides in a polyacrylamide gel are reported. Furthermore, a new preparative method to purify and collect oligonucleotides is presented. Separation performance of the gels was tested with various samples of oligonucleotides.

📜 SIMILAR VOLUMES

Preparation of polyacrylamide gel-filled

Preparation of polyacrylamide gel-filled capillaries for capillary electrophoresis

✍ Vladislav Dolnik; Kelly A. Cobb; Milos Novotny 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 427 KB

## Abstract A procedure for preparing polyacrylamide gel‐filled capillaries, for use in capillary gel electrophoresis, is decribed. The method involves the principle of isotachophoretic polymerization, in which the polymerization initiator is introduced by electromigration to promote gradual polyme

Production of polyacrylamide gel filled

Production of polyacrylamide gel filled capillaries for capillary gel electrophoresis (CGE): Influence of capillary surface pretreatment on performance and stability

✍ Yin, Hong-Feng ;Lux, Jürgen A. ;Schomburg, Gerhard 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 406 KB

## Abstract Polyacrylamide gel filled capillaries for electrophoresis can be improved in performance and stability by a specially optimized and reproducible production procedure. After surface pretreatment as described in this contribution, gels polymerized in capillaries are stable for long series

Formamide modified polyacrylamide gels f

Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis

✍ Marie J. Rocheleau; Ronda J. Grey; Da Yong Chen; Heather R. Harke; Norman J. Dov 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB

A simple method for the production of ge

A simple method for the production of gel-filled capillaries for capillary gell electrophoresis

✍ Lux, Jürgen A. ;Yin, Hong-Feng ;Schomburg, Gerhard 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 209 KB

Calibration of polyacrylamide gel column

Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis

✍ Dr. Aran Paulus; Ernst Gassmann; Matthew J. Field 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 741 KB

Band broadening of DNA fragments isolate

Band broadening of DNA fragments isolated by polyacrylamide gel electrophoresis in capillary electrophoresis

✍ Takashi Kaneta; Takehito Ogura; Shuhei Yamato; Totaro Imasaka 📂 Article 📅 2012 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB

## Abstract Polyacrylamide gel electrophoresis (PAGE) is used frequently for isolation and purification of DNA fragments. In the present study, DNA fragments extracted from polyacrylamide gels showed significant band broadening in capillary electrophoresis (CE). A pHY300PLK (a shuttle vector functi