Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis
β Scribed by Dr. Aran Paulus; Ernst Gassmann; Matthew J. Field
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 741 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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## Abstract A new preparation method for polyacrylamide gels in fused silica capillaries is described. In this method use is made of riboflavin as a light sensitive initiator for the polymerization reaction. Also, results from measurements on the diffusion coefficients of four oligonucleotides in a
## Abstract A procedure for preparing polyacrylamide gelβfilled capillaries, for use in capillary gel electrophoresis, is decribed. The method involves the principle of isotachophoretic polymerization, in which the polymerization initiator is introduced by electromigration to promote gradual polyme