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Preparation of polyacrylamide gel-filled capillaries for capillary electrophoresis

โœ Scribed by Vladislav Dolnik; Kelly A. Cobb; Milos Novotny

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
427 KB
Volume
3
Category
Article
ISSN
1040-7685

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โœฆ Synopsis

Abstract

A procedure for preparing polyacrylamide gelโ€filled capillaries, for use in capillary gel electrophoresis, is decribed. The method involves the principle of isotachophoretic polymerization, in which the polymerization initiator is introduced by electromigration to promote gradual polymerization of the acrylamide in a sequential manner along the length of the capillary. This procedure has been found to be superior to other methods for polymerizing gels inside of smallโ€bore (i.e., 50โ€ฮผm i.d.) capillaries, because of the reduced formation of bubbles in the gels during polymerization. The method described is simple, reproducible, and several capillaries can be prepared simultaneously. A preliminary result showing the use of 50โ€ฮผm i. d. gelโ€filled capillaries for SDSโ€polyacrylamide gel electrophoresis of proteins is shown.

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