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Production of polyacrylamide gel filled capillaries for capillary gel electrophoresis (CGE): Influence of capillary surface pretreatment on performance and stability

✍ Scribed by Yin, Hong-Feng ;Lux, Jürgen A. ;Schomburg, Gerhard

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
406 KB
Volume
13
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

Polyacrylamide gel filled capillaries for electrophoresis can be improved in performance and stability by a specially optimized and reproducible production procedure. After surface pretreatment as described in this contribution, gels polymerized in capillaries are stable for long series of routine separations. Bubbles formation during gel polymerization can be avoided. The procedure is compared with those described by other authors. Separations of different oligonucleotide samples illustrate the quality of the gel capillaries obtained. The peak symmetry achieved proved to be better than that obtained without surface pretreatment.

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