## Abstract A procedure for preparing polyacrylamide gelβfilled capillaries, for use in capillary gel electrophoresis, is decribed. The method involves the principle of isotachophoretic polymerization, in which the polymerization initiator is introduced by electromigration to promote gradual polyme
Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis
β Scribed by Marie J. Rocheleau; Ronda J. Grey; Da Yong Chen; Heather R. Harke; Norman J. Dovichi
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 321 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Polyacrylamide gel electrophoresis (PAGE) is used frequently for isolation and purification of DNA fragments. In the present study, DNA fragments extracted from polyacrylamide gels showed significant band broadening in capillary electrophoresis (CE). A pHY300PLK (a shuttle vector functi
## Abstract Polyacrylamide gels are unstable in 50βΞΌm internal diameter capillaries at electric fields greater than about 500 V cm^β1^ due to the formation of bubbles, which destroy the separation. We describe the use of a modified polyacrylamide capillary gel that is stable at electric fields of a
## Abstract Polyacrylamide gel filled capillaries for electrophoresis can be improved in performance and stability by a specially optimized and reproducible production procedure. After surface pretreatment as described in this contribution, gels polymerized in capillaries are stable for long series