## Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. P
Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments
✍ Scribed by Hildegard Haas; Bruce Budowle; Günter Weiler
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 528 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0173-0835
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## Abstract Polyacrylamide gel electrophoresis (PAGE) is used frequently for isolation and purification of DNA fragments. In the present study, DNA fragments extracted from polyacrylamide gels showed significant band broadening in capillary electrophoresis (CE). A pHY300PLK (a shuttle vector functi
A technique for rapidly and quantitatively denaturing double-stranded DNA employing urea and moderate heat is described. The single DNA strands are resolved on high-percentage nondenaturing polyacrylamide gels from which they can be recovered for Maxam-Gilbert sequence analysis.
PAGE 3 systems capable of separating peptides of M r below 10,000 have been reported. Most of these methods have used separation gels with urea (1-4), high concentrated and crosslinked polyacrylamide (5), or a steep polyacrylamide gradient (6, 7) in order to achieve successful resolution of the pept