𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Plasmid transformation of competent Bacillus subtilis by lysed protoplast DNA

✍ Scribed by Takashi Akamatsu; Hisataka Taguchi

Book ID
116616671
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
241 KB
Volume
114
Category
Article
ISSN
1389-1723

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

High frequency transformation of Bacillu
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
✍ Chang, Shing ;Cohen, Stanley N. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra

Fate of plasmid DNA in transformation of
Fate of plasmid DNA in transformation of Bacillus subtilis protoplasts
✍ de Vos, Willem M. ;Venema, Gerard πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 603 KB

Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the