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Physiological studies on pBR 322 DNA amplification in an Escherichia coli relA strain

✍ Scribed by Dr. M. Hecker; A. Schroeter; F. Mach

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 349 KB
Volume: 26
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620260605

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✦ Synopsis

Amino acid limitation leads in E. coli relA cells which cannot synthesize guanosine tetraphosphate (ppGpp) under these conditions to an amplification of pBR 322 DNA. We previously proposed that ppGpp produced in E . coli relA+ cells subjected to amino acid limitation inhibits pBR 322 DNA replication (HECKER et al. 1983).

I n further experiments i t was established that an E . coZi reZA strain shows plasmid amplification during amino acid limitation (arginine, threonine, leucine or histidine) only in the presence of sufficient concentrations of phosphate, ammonia and glucose. Plasmid amplification does not occur if ammonia or phosphate is depleted. We suggest that glucose, ammonia and phosphate are needed for nucleotide and deoxynucleotide synthesis as the essential prerequisite for plasmid amplif ication .

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