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Replication of pBR322 DNA in stringent and relaxed strains of Escherichia coli

✍ Scribed by Hecker, Michael ;Schroeter, Andreas ;Mach, Friedrich

Publisher: Springer
Year: 1983
Tongue: English
Weight: 392 KB
Volume: 190
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00330665

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✦ Synopsis

Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA+:CP78; relA:CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated only in the relaxed strain and was inhibited in its stringent counterpart. The results suggest that replication of plasmid DNA is negatively controlled by the relA+ allele.

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