𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of relA function on the replication of plasmid pBR322 in Escherichia coli

✍ Scribed by Lin-Chao, S. ;Bremer, H.


Publisher
Springer
Year
1986
Tongue
English
Weight
325 KB
Volume
203
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Role of DnaA protein during replication
✍ Chiang, C. -S. ;Xu, Y. -C. ;Bremer, H. πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 948 KB

The in vivo role of the Escherichia coli protein DnaA in the replication of plasmid pBR322 was investigated, using a plasmid derivative carrying an inducible dnaA+ gene. In LB medium without inducer, the replication of this plasmid, like that of pBR322, was inhibited by heat inactivation of chromoso

Amplification of pBR322 plasmid DNA in E
✍ Dr. K. H. Hofmann; P. Neubauer; Sabine Riethdorf; M. Hecker πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 298 KB

## Abstract Fermenter studies under batch and fed‐batch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using __E. coli relA__ strains. High amplification rates of pBR322 plasmid DNA were observed in __E. coli__ CP79 (__relA__) and __E. coli__ CP

Replication of pBR322 DNA in stringent a
✍ Hecker, Michael ;Schroeter, Andreas ;Mach, Friedrich πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 392 KB

Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA+:CP78; relA:CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated o

Rifampicin-induced replication of the pl
✍ Polaczek, Piotr ;CieΕ›la, Zygmunt πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 759 KB

The replication pattern of the plasmid pBR322 was examined in the dnaA mutants of Escherichia coli. The rate of pBR322 DNA synthesis is markedly decreased after dnaA cells are shifted to the restrictive temperature of 42 degrees C. However, addition of rifampicin (RIF) to cultures of dnaA strains in