The cloning of Aspergillus nidulans mitochondrial DNA in Escherichia coli on plasmid pBR322
✍ Scribed by Bartnik, Ewa ;Biderman, Andrzej W. ;Hahn, Ulrich ;Küntzel, Hans ;Stpień, Piotr P.
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 636 KB
- Volume
- 182
- Category
- Article
- ISSN
- 0026-8925
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## Abstract Fermenter studies under batch and fed‐batch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using __E. coli relA__ strains. High amplification rates of pBR322 plasmid DNA were observed in __E. coli__ CP79 (__relA__) and __E. coli__ CP
The 4.2 Md EcoRI fragment of Aspergillus nidulans mitochondrial DNA was cloned using the plasmid pBR 332 as vector and E. coli as host. Hitherto unknown sequence of HindIII sites within this region of mitochondrial genome was established.